Lipase Assay

Available online at www.pelagi aresearchlibrary.com Pelagia Research Library Advances in utilise comprehension Research, 2011, 2 (2): 265-271 ISSN: 0976-8610 CODEN (USA): AASRFC Aqueous deuce phase rip of Lipase from sift Bran Mukesh Kumar Sharma Institute of Biotechnology and Allied Sciences, Piprali Road, Sikar (Rajasthan) ______________________________________________________________________________ ABSTRACT out-of-pocket to heterogeneous application of lipase in medical and industrial field, aqueous two-phase stock for the downstream processing of lipase has been exploited. The influence of establishment parameters such as phase forming salts, molecular weight of the phase forming polymer, system pH, inter-group communication line length, and phase volume ratio on the partition off behavior of lipase was evaluated. The aqueous two-phase system consisting of nog 4000 and Magnesium sulfate and it has shown better results with the purification of 1.648 fold . As nail 4000 with Magnesium sulphate has shown better results so further score can be carried out using PEG 4000 as phase forming polymer. Keywords: crystallineliquid extraction - Lipase - Aqueous two phase extraction - Partition coefficient, Lipase, Rice Bran.

______________________________________________________________________________ INTRODUCTION Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are ubiquitous enzymes of large physiological significance and industrial potential. Lipase change state the hydrolysis of triacylglcerols to glycerol and withdraw fatty acids at oil-water interface. They have protracted application in food, pharmaceutical, paper, cosmetic, detergen t, and leather industries. Recent developmen! ts in biotechnology have loose up new avenues towards the production of more enzymes/proteins of important in research, pharmaceutical and industrial applications. There has been an increase interest in the development of efficient down stream processing methods for separation, assimilation and purification of...If you want to get a full essay, rove it on our website: OrderEssay.net

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